MtbDsigH

The parental candidate is an isogenic mutant in Mtb CDC1551 gene Rv3223c (sigH). We have now generated multiple Mtb double and triple knock-out (DKO, TKO) strains including: 1. DsigHDsecA2DsodA 2. DsigHDfbpADsapM 3. DsigHDleuCDpanCD 4. DsigHDmetA 5. DsigHDmce4EDmce4F 6. DsigHDfadD29 7. DsigHDhadC 8. DsigHDRv3683 9. DsigHDmce1A 10. DsigHDcobM

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BCG::ESAT6-PE25SS

BCG::ESAT6-PE25SS is a recombinant BCG strain generated on the Danish background. The strain was constructed by fusing the ESAT-6-encoding gene esxA to the general secretion signal for the mycobacterial type VII secretion pathway protein PE25. BCG::ESAT6-PE25SS secretes full-length ESAT-6 via the ESX-5 secretion system, which in contrast to ESX-1 is also present in BCG.

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Mycobacterium microti ATCC 35782

The Mycobacterium microti ATCC 35782 strain corresponds to the M. microti strain used in Prague in the 1960’s where it was known as the ‘M.P.’ strain, referring to the abbreviated form of ‘Murinus Praha’. The M.P. strain originates from Dr Wells’ OV166 strain, isolated from Orkney voles [3], which was then adapted by Dr Sula…

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BCG::ESX-1Mmar

BCG::ESX-1Mmar is a recombinant BCG strain into which the extended region of the ESX-1-encoding genomic region of Mycobacterium marinum was integrated.

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