Mycobacterium microti ATCC 35782
Candidate Overview
The Mycobacterium microti ATCC 35782 strain corresponds to the M. microti strain used in Prague in the 1960’s where it was known as the ‘M.P.’ strain, referring to the abbreviated form of ‘Murinus Praha’. The M.P. strain originates from Dr Wells’ OV166 strain, isolated from Orkney voles [3], which was then adapted by Dr Sula to fully synthetic medium and used for preparation of the different vaccine lots for human vaccination (see References under Additional Information).
Sponsor / Lead Developer: Institut Pasteur Paris
Primary Indication: Prevention of TB disease
Target Population(s): Elderly and People living with HIV
Target Route of Administration: Intradermal
Immune tissue localization: Lung and Lymph node
Immunological responses: T-cell
Preclinical Animal Models: Guinea pig and Mouse
Intended to elicit trained immunity: Yes
Additional Immunologic Response Information
HYPOTHESIZED | DEMONSTRATED |
|
| Immune Response | T-cell | |
| T-cell phenotype | CD4 | |
| T-cell functional profile | IFN-γ | |
| Preferential immune tissue localization | Lung Lymph node | |
| Trained immunity | Yes |
Related Publications
Additional Information
We have recently tested this vaccine in a SCID mouse model and found that the Mycobacterium microti ATCC 35782 strain was completely inoffensive in this highly susceptible model (SCID mice still gained weight 21 weeks after infection while BCG-infected mice had all reached their humane endpoint by week 17). However, when tested for vaccine efficacy in a guinea pig model, the M. microti strain showed protection against a challenge with virulent M. tuberculosis that was similar to BCG-induced protection (Orgeur et al., Microbial Genomics 000505, DOI 10.1099/mgen.0.000505
References
Sula L, Radkovský I. Protective effects of M. microti vaccine against tuberculosis. J Hyg Epidemiol Microbiol Immunol 1976;20:1–6. Reference
Sula L, Zavadilova Z, Medulanova L, Pokorny J. [New vaccine against tuberculosis. II. Characteristics of the strain, Mycobacterium tuberculosis, murine type-Wells OV 166, and preparation of the vaccine]. Cas Lek Cesk 1952;91:161–171.