MtbΔfbpA
Candidate Overview
We have developed three live vaccine candidates by knocking out or deleting genes in Mycobacterium tuberculosis H37Rv. We named them as double knockout (DKO), Triple knockout (TKO), and quadruple knockout (QKO). These strains have the following gene deletions: DKO (Mtb∆fbpA-∆sapM), TKO (Mtb∆fbpA-∆sapM-∆zmp1), and (Mtb∆fbpA-∆sapM-∆zmp-∆dosR).
Sponsor / Lead Developer: The University of Texas Rio Grande Valley
Development partner(s): Texas Tech University Health Sciences Center, Houston Methodist Research Institute
Primary Indication: Prevention of Mtb infection or sustained infection
Other Indication(s): Prevention of TB disease
Target Population(s): To be determined
Target Route of Administration: Subcutaneous
Immunological responses: T-cell
Preclinical Animal Models: Mouse
Additional Immunologic Response Information
HYPOTHESIZED | DEMONSTRATED |
|
| Immune Response | T-cell | |
| T-cell phenotype | CD4 CD8 |
|
| T-cell functional profile | IFN-γ TNF-α IL-17 | |
| Preferential immune tissue localization | Lung | |
| Trained immunity | No | |
Related Publications
Comparative transcriptomic analysis of mouse macrophages infected with live attenuated vaccine strains of Mycobacterium tuberculosis (Front Immunol. 2025)
The ΔfbpAΔsapM candidate vaccine derived from Mycobacterium tuberculosis H37Rv is markedly immunogenic in macrophages and induces robust immunity to tuberculosis in mice. (Front. Immunol. 2024)
The fbpA/sapM Double Knock Out Strain of Mycobacterium tuberculosis Is Highly Attenuated and Immunogenic in Macrophages(PLOS One, 2012)
Additional Information
A manuscript describing the immunogenicity and efficacy of TKO and QKO strains has been submitted for publication in the journal “Infection and Immunity#.